Short Lecture : The secretory pathway of Pichia pastoris: genomics, regulation and redox metabolism
The availability of genome sequences and genome-scale regulatory data enables a deeper look into the actual cellular processes of non-conventional yeasts. Based on a comprehensive analysis of all secretory pathway genes in Saccharomyces cerevisiae and 7 other yeast species main differences in genetic setup between baker’s yeast and Pichia pastoris will be presented. We have studied the regulation of all secretion related genes upon a broad range of specific growth rates revealing opposite regulatory trends for different sub-pathways. As an example, N- and O-glycosylation will be discussed in detail on a genomic and transcriptomic level. Finally the impact of redox processes and their regulation on protein folding and secretion will be discussed. The highlighted genomic setup of P. pastoris and its regulation define the frame of our continuing effort to engineer the secretory pathway towards high efficiency.