ABOUT PICHIA 2021

A virtual conference | Starting March 24, 2021
15:00 to 19:00 GMT

Want to see what time the sessions are hosted in your time zone? Use this helpful time zone converter .

Hosted by BioGrammatics and Research Corporation Technologies
and focused on recombinant protein expression with the yeast Pichia pastoris (Komagataella)

ORGANIZERS

OUR SPONSORS

As a virtual conference, Pichia 2021, will be different than any previous Pichia conference, with both advantages and challenges to support the Pichia community. We are excited to interact with you and other “ Pichia people” virtually, but also hope to see you "in person", asap (perhaps Pichia 2022 - Asilomar - California).

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Program

Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Posters

TITLE:	DATE/TIME:	LOCATION:
Day 1 : Introduction to the Pichia pastoris Expression System.	Mar 24, 2021 3:15 PM - 3:45 PM GMT	Virtual
Day 1 : Eptinezumab - The discovery and development of the first FDA approved Full-length Pichia produced Monoclonal Antibody.	Mar 24, 2021 3:45 PM - 4:15 PM GMT	Virtual
Day 1 : Engineering Pichia pastoris to make the Impossible burger possible.	Mar 24, 2021 4:15 PM - 4:45 PM GMT	Virtual
Day 1 : CO2 assimilation and conversion with Pichia.	Mar 24, 2021 5:00 PM - 5:30 PM GMT	Virtual
Day 1 : Investigation of alternative methanol assimilation in Komagataella phaffii.	Mar 24, 2021 5:30 PM - 6:00 PM GMT	Virtual
Day 1 : Expression and characterization of biologically active glycoprotein hormones using the Pichia GlycoSwitch® technology.	Mar 24, 2021 6:00 PM - 6:30 PM GMT	Virtual
Day 1 : Bioprocess development through AI Models to control cell physiology in Pichia pastoris recombinant protein production.	Mar 24, 2021 6:30 PM - 7:00 PM GMT	Virtual

TITLE:	DATE/TIME:	LOCATION:
Day 2 : Pichia antibodies against infectious diseases of respiratory and GI tract.	Mar 25, 2021 3:15 PM - 3:45 PM GMT	Virtual
Day 2 : Pichia, an expression platform for developing malaria vaccines.	Mar 25, 2021 3:45 PM - 4:15 PM GMT	Virtual
Day 2 : Exploiting Pichia pastoris N-glycosylation machinery to design more versatile camelid single domain antibodies (VHHs).	Mar 25, 2021 4:15 PM - 4:45 PM GMT	Virtual
Day 2 : Engineering molecules and hosts for biopharmaceuticals and vaccines.	Mar 25, 2021 5:00 PM - 5:30 PM GMT	Virtual
Day 2 : SARS‑CoV‑2‑spike receptor binding domain produced in Pichia pastoris and mammalian cells. Uses in diagnosis, in obtaining neutralizing antibodies and as a potential vaccine.	Mar 25, 2021 5:30 PM - 6:00 PM GMT	Virtual
Day 2 : Development of a cost-effective process for the heterologous production of SARS-CoV2 spike receptor binding domain (RBD) using Pichia pastoris.	Mar 25, 2021 6:00 PM - 6:30 PM GMT	Virtual
Day 2 : Methanol-free expression of SARS-CoV2-RBD variants in Pichia pastoris.	Mar 25, 2021 6:30 PM - 7:00 PM GMT	Virtual

TITLE:	DATE/TIME:	LOCATION:
Day 3 : Improved “CRISPR technologies” in Pichia/Komagatella.	Mar 26, 2021 3:15 PM - 3:45 PM GMT	Virtual
Day 3 : Versatile role of the FLO gene family and Implications for recombinant protein secretion, gene regulation and cell morphology.	Mar 26, 2021 3:45 PM - 4:15 PM GMT	Virtual
Day 3 : Silk secretion in Pichia.	Mar 26, 2021 4:15 PM - 4:45 PM GMT	Virtual
Day 3 : Engineering a novel Pichia pastoris cell-free protein synthesis platform.	Mar 26, 2021 5:00 PM - 5:30 PM GMT	Virtual
Day 3 : Establishing Pichia pastoris as a promising candidate for bulk chemicals production: the 3-hydroxypropionic acid case.	Mar 26, 2021 5:30 PM - 6:00 PM GMT	Virtual
Day 3 : Pichia pastoris directly delivers functional recombinant proteins to cultured mammalian cells.	Mar 26, 2021 6:00 PM - 6:30 PM GMT	Virtual
Day 3 : Quantitative Trait Loci for protein secretion in Komagataella phaffii.	Mar 26, 2021 6:30 PM - 7:00 PM GMT	Virtual

TITLE:	DATE/TIME:	LOCATION:
Day 4 : Bioprocessing with Pichia pastoris Production of recombinant pharmaceutical proteins and peptides.	Mar 29, 2021 4:15 PM - 4:45 PM BST	Virtual
Day 4 : Recombinant protein production in Pichia pastoris; alternative methanol-free expression systems for industrial implementation.	Mar 29, 2021 4:45 PM - 5:15 PM BST	Virtual
Day 4 : Improvement of bioprocess efficiency in Pichia pastoris recombinant protein production combining gene dosage and oxygen limitation.	Mar 29, 2021 5:15 PM - 5:45 PM BST	Virtual
Day 4 : Automated methanol induction and low pH profiling in a microbioreactor.	Mar 29, 2021 6:00 PM - 6:20 PM BST	Virtual
Day 4 : High cell density cultivations of Pichia pastoris in microbioreactor system.	Mar 29, 2021 6:20 PM - 6:40 PM BST	Virtual
Day 4 : Recombinant production of peptide-based antibiotics using P. pastoris as host-expression system Part I: Design and proof of principle.	Mar 29, 2021 6:40 PM - 7:00 PM BST	Virtual
Day 4 : Recombinant production of peptide-based antibiotics using P. pastoris as host-expression system Part II: Fully automated three stage upstream scale up process.	Mar 29, 2021 7:00 PM - 7:20 PM BST	Virtual
Day 4 : Extractive fermentation in Aqueous Two-phase Systems (ATPS) to produce recombinant proteins in Pichia pastoris.	Mar 29, 2021 7:20 PM - 7:40 PM BST	Virtual

TITLE:	DATE/TIME:	LOCATION:
Day 5 : Selective autophagy - insights from studying pexophagy using Pichia pastoris.	Mar 30, 2021 4:15 PM - 4:45 PM BST	Virtual
Day 5 : Komagataella phaffii Cue5 piggybacks on lipid droplets for its vacuolar degradation.	Mar 30, 2021 4:45 PM - 5:15 PM BST	Virtual
Day 5 : Komagataella phaffii eIF2A suppresses the selective autophagy of lipid droplets.	Mar 30, 2021 5:15 PM - 5:45 PM BST	Virtual
Day 5 : Autophagy is responsible for degradation of the cytosolic enzymes of methanol metabolism in Komagataella phaffii (Pichia pastoris).	Mar 30, 2021 6:00 PM - 6:30 PM BST	Virtual
Day 5 : Understanding the role of autophagy during metabolic adaptation in the methylotrophic yeast, Pichia pastoris.	Mar 30, 2021 6:30 PM - 7:00 PM BST	Virtual
Day 5 : All yeasts are not created equal: Examining Golgi stacking in Pichia pastoris.	Mar 30, 2021 7:00 PM - 7:30 PM BST	Virtual
Day 5 : Intro to Poster Session	Mar 30, 2021 7:30 PM - 7:45 PM BST	Virtual

TITLE:	DATE/TIME:	LOCATION:
Day 6 : New Methanol Free Production Strain and Process Development.	Mar 31, 2021 4:15 PM - 4:45 PM BST	Virtual
Day 6 : Methanol-induced gene expression: molecular mechanism and application.	Mar 31, 2021 4:45 PM - 5:15 PM BST	Virtual
Day 6 : Screening and characterization of novel strong promotors by RNA sequencing and RT-qPCR for recombinant protein production in Pichia pastoris.	Mar 31, 2021 5:15 PM - 5:45 PM BST	Virtual
Day 6 : Making a strong promoter even stronger - Enhancing transcriptional activity of the glucose-regulated PG1 promoter by random mutagenesis and transcriptional engineering.	Mar 31, 2021 5:45 PM - 6:05 PM BST	Virtual
Day 6 : Lonza’s XS® Pichia 2.0 – redefining upstream process development	Mar 31, 2021 6:20 PM - 6:40 PM BST	Virtual
Day 6 : Fast track development with Pichia pastoris – meeting the challenges of protein production using UNLOCK PICHIA.	Mar 31, 2021 6:40 PM - 7:00 PM BST	Virtual
Day 6 : Enhanced Biomanufacturing of Novel Antibiotics and Vaccines in Pichia pastoris.	Mar 31, 2021 7:00 PM - 7:20 PM BST	Virtual
Day 6 : Pitfalls and Solutions in Pichia Bioprocessing.	Mar 31, 2021 7:20 PM - 7:40 PM BST	Virtual

TITLE:	DATE/TIME:	LOCATION:
Day 7 : Glycosylation by Pichia pastoris improved the recombinant Endoglucanase D thermal stability.	Apr 01, 2021 4:15 PM - 4:45 PM BST	Virtual
Day 7 : Production of secreted plant and fungal pathogen proteins in Pichia pastoris.	Apr 01, 2021 4:45 PM - 5:00 PM BST	Virtual
Day 7 : Intracellular recombinant production of an enzyme to treat vision impairment.	Apr 01, 2021 5:00 PM - 5:45 PM BST	Virtual
Day 7 : Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory system transit.	Apr 01, 2021 6:00 PM - 6:20 PM BST	Virtual
Day 7 : Yeast-based single domain antibody platform for rapid response to pandemic outbreaksVIB-UGent Center for Medical Biotechnology.	Apr 01, 2021 6:20 PM - 6:40 PM BST	Virtual
Day 7 : A CRISPR/Cas9 based tool for precise oligonucleotide-mediated gene editing in Komagataella phaffii.	Apr 01, 2021 6:40 PM - 7:00 PM BST	Virtual
Day 7 : MicroNAP: A framework to characterize genetic changes in engineered microbial strains based on Next Generation Sequencing data.	Apr 01, 2021 7:00 PM - 7:20 PM BST	Virtual

TITLE:	SPEAKERS:	DATE/TIME:	LOCATION:
Posters : P01: A fragment of the prosequence of Rhizopus oryzae lipase, key factor for enzyme production and its operational stability		Mar 30, 2021 12:00 PM - 12:05 PM BST
Posters : P02: Fermentation development of Pfs230D1M, a transmission blocking malaria vaccine candidate, in miniaturized bioreactors		Mar 30, 2021 12:05 PM - 12:10 PM BST
Posters : P03: Process Development Strategies for Improved Production of rhGM-CSF in Pichia pastoris through derepression of pFMD		Mar 30, 2021 12:10 PM - 12:15 PM BST
Posters : P05: Treatment with surfactants enables quantification of translational activity by O-propargyl-puromycin labelling in P. pastoris		Mar 30, 2021 12:15 PM - 12:12 AM BST
Posters : P06: Pichia pastoris as a versatile host for recombinant production of antifungal peptide ‘’PAF’’ from Penicillium chrysogenum		Mar 30, 2021 12:20 PM - 12:25 PM BST
Posters : P08: Komagataella phaffii as a model for selective autophagy of glycogen granules		Mar 30, 2021 12:25 PM - 12:30 PM BST
Posters : P09: Production of 3-hydroxypropionic acid from glycerol, methanol and fatty acids in Pichia pastoris: Towards the use of crude glycerol as a carbon source		Mar 30, 2021 12:30 PM - 12:35 PM BST
Posters : P10: Identification of genes involved in autophagic degradation of soluble proteins in Pichia pastoris		Mar 30, 2021 12:35 PM - 12:40 PM BST
Posters : P11: Constant feeding technology in Pichia pastoris clone screening allows to increase results robustness		Mar 30, 2021 12:40 PM - 12:45 PM BST
Posters : P12: Towards an accessible and non-restrictive Komagataella phaffii protein expression toolkit		Mar 30, 2021 12:45 PM - 12:50 PM BST
Posters : P13: Scale-dependent effect of helper factor co-expression in Pichia pastoris		Mar 30, 2021 12:50 PM - 12:55 PM BST
Posters : P14: A unique enzyme from endangered species expressed in Pichia Pastoris		Mar 30, 2021 12:55 PM - 1:00 AM BST
Posters : P15: Comparison of integrative vs. episomal CalB expression in K. phaffi using Gut1 as selection marker		Mar 30, 2021 1:00 PM - 1:05 PM BST
Posters : P17: Evaluating the efficiency of CRISPR/MAD7 genome editing system in Komagataella phaffii		Mar 30, 2021 1:05 PM - 1:10 PM BST
Posters : P18: Komagataella phaffii genome engineering employing RNA guided MAD7 nuclease		Mar 30, 2021 1:10 PM - 1:15 PM BST
Posters : P19: Optimization of culture conditions to increase phytase volumetric productivity in Pichia pastoris under the GAP promoter and impact on the physiological response of the host		Mar 30, 2021 1:15 PM - 1:20 PM BST
Posters : P20: Implementation of dMAD7 in Komagataella phaffii		Mar 30, 2021 1:20 PM - 1:25 PM BST
Posters : P21: Screening non-conventional yeasts for heterologous protein production capability by plasmid-based expression		Mar 30, 2021 1:25 PM - 1:30 PM BST
Posters : P22: Production of an active cellulose dehydrogenase (CDH) in Pichia pastoris expression system		Mar 30, 2021 1:30 PM - 1:35 PM BST
Posters : P23: Secretory Production of novel Peroxygenases in Pichia pastoris		Mar 30, 2021 1:35 PM - 1:40 PM BST
Posters : P24: Novel Pichia pastoris platform strain for the expression of secreted oxidative enzymes - from “Jackpot” clone to microbial chassis		Mar 30, 2021 1:40 PM - 1:45 PM BST
Posters : P25: Enzyme promiscuity enables efficient recombinant protein production, on methanol, in an AOX deficient Pichia pastoris strain		Mar 30, 2021 1:45 PM - 1:50 PM BST
Posters : P26: Identification of major kinase genes in the first step of glycolysis of Pichia pastoris		Mar 30, 2021 1:50 PM - 1:55 PM BST
Posters : P27: Development of a high productivity process for phospholipase production in Pichia pastoris		Mar 30, 2021 1:55 PM - 2:00 PM BST
Posters : P28: Expression and Production of Extended SBLE in Pichia pastoris: A mutant of an Unusual Esterase		Mar 30, 2021 2:00 PM - 2:05 PM BST
Posters : P29: Simultaneous inactivation of two genes in Komagataella phaffii via CRISPR/Cas9 mediated gene disruptions		Mar 30, 2021 2:05 PM - 2:10 PM BST
Posters : P30: Transcriptomic-guided development of Pichia pastoris strains with improved methanol-free productivity		Mar 30, 2021 2:10 PM - 2:15 PM BST
Posters : P31: Production in semi-continuous mode with the yeast Pichia pastoris using BIONET’s F1 bioreactor		Mar 30, 2021 2:15 PM - 2:18 PM BST
Posters : P32: Manufacturing and assembly of human vault particles in Pichia pastoris for therapeutic applications		Mar 30, 2021 2:18 PM - 2:20 PM BST
Posters : P33: Application of the Golden PiCS vector system for generation of combinatorial libraries of Yap1 regulated genes		Mar 30, 2021 2:20 PM - 2:25 PM BST
Posters : P36: Pichia pastoris: QTL mapping for strain development		Mar 30, 2021 2:25 PM - 2:30 PM BST
Posters : P37: A synthetic Calvin cycle enables autotrophic growth in yeast		Mar 30, 2021 2:30 PM - 2:35 PM BST
Posters : P38: Functional characterization of a novel non-methanol dependent Pichia pastoris promoter		Mar 30, 2021 2:35 PM - 2:40 PM BST
Posters : P39: PRODUCTION OF HUMAN EPIDERMAL GROWTH FACTOR (HEGF) IN KOMAGATAELLA PHAFFII		Mar 30, 2021 2:40 PM - 2:45 PM BST
Posters : P40: Cloning and functional characterization of Komagataella phaffii centromeric sequences		Mar 30, 2021 2:45 PM - 2:50 PM BST
Posters : P41: NON-INVASIVE BIOMASS MEASUREMENTS FOR PICHIA PASTORIS IN SHAKE FLASK AND BIOREACTOR CULTIVATIONS		Mar 30, 2021 2:50 PM - 2:55 PM BST
Posters : P42: Protein Production in Pichia: High yield and Low O-glycosylation Platform		Mar 30, 2021 2:55 PM - 3:00 PM BST

SUBMIT YOUR ABSTRACT

ABSTRACTS SUBMISSION IS STILL OPEN

The opening sessions are full, however, abstracts are still accepted for subsequent sessions and posters.

SUBMIT YOUR ABSTRACT

The virtual conference will start with March 24, 25 and 26, 2021, from 3:00 p.m. to 7:00 p.m. GMT, and subsequent sessions will be added depending on the number of abstracts received and interest in subsequent sessions.

Individual presentations are ideally 20 to 30 minutes. Presentations can be livestreamed or pre-recorded, or a combination of both. Preference will be given to presenters who can attend their presentation with some live presence. For example, an introduction and answering questions at the end of the presentation.

Most any presentation on research and scientific applications of Pichia pastoris will be considered, including presentations on:

1) general biology,

2) process development/fermentation,

3) "Omic" characterization (e.g. genome, transcriptome, proteome).

4) applications, trouble shooting and success stories.

5) biocatalysts.

Instructions will be provided on presentation format options for your "talk" or "poster" once selected for the program. We will try to be as flexible as possible. More than one submission per person is welcome. There is no limit to the number of posters; most any scientific presentation will be accepted.

IMPORTANT DEADLINES:

- Submission Deadline: Opening session closed. Abstract submission for posters and subsequent sessions is still open; abstracts submitted after March 10th are not guaranteed to be available for the opening sessions.

- Review period: Ongoing

- Program Announced: February 20, 2021

Questions? Please contact us at info@pichia2021.com .

SUBMITTING YOUR PRESENTATIONS

TALKS
Pre-recorded presentations/videos, must be submitted in mp4 format, < 3.5GB in file size.
Please use the speakers name as the name of the video file, followed by the Day of the talk (Day 1-7, as in Program), as follows: SPEAKER NAME-DayX-Pichia2021.mp4
Video submission deadline Friday, March 19 13:00 GMT.
Upload Talk files here .

Talks presented "Live" will be streamed from the slides from the presenter's computer, these slides can be uploaded to the conference site as PDF files (i.e. as a backup for event, and also to post to the conference site). Please upload as: SPEAKER NAME-DayX-Pichia2021.PDF here .

POSTERS

Posters can be submitted in PDF format, or if as a video in mp4 format (file size < 3.5GB). Submit single slide deck "posters", or slide decks as a PDF file.

For submission, use the presenters in the file name, for example a video poster would be: SPEAKER NAME-Poster-Pichia2021.MP4
A PDF file would be: SPEAKER NAME-Poster-Pichia2021.PDF.

To guarantee your poster is available for opening day, the submission deadline is Friday, March 19, 13:00 GMT. Upload Poster files here .

REGISTRATION

Student: $50US
Academic: $150US
Industry: $250US

Use discount code PICHIA50 to save $50 on your registration as a speaker or poster presenter.

Scholarships and group rates available. Contact info@pichia2021.com to inquire.

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CONTACT US

info@pichia2021.com

Student	$50.00
Academic	$150.00
Industry	$250.00